Deconstructing the Polymerase Chain Reaction II: an improved workflow and effects on artifact formation and primer degeneracy

Polymerase chain reaction (PCR) amplification of complex microbial genomic DNA templates with degenerate primers can lead to distortion of the underlying community structure due to inefficient primer-template interactions leading to bias. We previously described a method of deconstructed PCR (“PEX P...

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Detalles Bibliográficos
Autores principales: Naqib, Ankur, Poggi, Silvana, Green, Stefan J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2019
Materias:
Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6573857/
https://www.ncbi.nlm.nih.gov/pubmed/31231597
http://dx.doi.org/10.7717/peerj.7121

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6573857/
https://www.ncbi.nlm.nih.gov/pubmed/31231597
http://dx.doi.org/10.7717/peerj.7121

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