A simplified transposon mutagenesis method to perform phenotypic forward genetic screens in cultured cells

BACKGROUND: The introduction of genome-wide shRNA and CRISPR libraries has facilitated cell-based screens to identify loss-of-function mutations associated with a phenotype of interest. Approaches to perform analogous gain-of-function screens are less common, although some reports have utilized arra...

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Detalles Bibliográficos
Autores principales: Feddersen, Charlotte R., Wadsworth, Lexy S., Zhu, Eliot Y., Vaughn, Hayley R., Voigt, Andrew P., Riordan, Jesse D., Dupuy, Adam J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580595/
https://www.ncbi.nlm.nih.gov/pubmed/31208320
http://dx.doi.org/10.1186/s12864-019-5888-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580595/
https://www.ncbi.nlm.nih.gov/pubmed/31208320
http://dx.doi.org/10.1186/s12864-019-5888-6

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