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A simplified transposon mutagenesis method to perform phenotypic forward genetic screens in cultured cells
BACKGROUND: The introduction of genome-wide shRNA and CRISPR libraries has facilitated cell-based screens to identify loss-of-function mutations associated with a phenotype of interest. Approaches to perform analogous gain-of-function screens are less common, although some reports have utilized arra...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6580595/ https://www.ncbi.nlm.nih.gov/pubmed/31208320 http://dx.doi.org/10.1186/s12864-019-5888-6 |