Effects of concentration of CRISPR/Cas9 components on genetic mosaicism in cytoplasmic microinjected porcine embryos

Cytoplasmic microinjection (CI) of the CRISPR/Cas9 system enabled the induction of site-specific mutations in porcine zygotes and resulting pigs. However, mosaicism is a serious problem for genetically modified pigs. In the present study, we investigated suitable timing and concentration of CRISPR/C...

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Detalles Bibliográficos
Autores principales: TANIHARA, Fuminori, HIRATA, Maki, NGUYEN, Nhien Thi, LE, Quynh Anh, HIRANO, Takayuki, OTOI, Takeshige
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Society for Reproduction and Development 2019
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6584178/
https://www.ncbi.nlm.nih.gov/pubmed/30726783
http://dx.doi.org/10.1262/jrd.2018-116

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6584178/
https://www.ncbi.nlm.nih.gov/pubmed/30726783
http://dx.doi.org/10.1262/jrd.2018-116

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