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The chromatin remodeler Chd1 regulates cohesin in budding yeast and humans

Chd1 is a chromatin remodeler that is involved in nucleosome positioning and transcription. Deletion of CHD1 is a frequent event in prostate cancer. The Structural Maintenance of Chromosome (SMC) complex cohesin mediates long-range chromatin interactions and is involved in maintaining genome stabili...

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Autores principales: Boginya, Alexandra, Detroja, Rajesh, Matityahu, Avi, Frenkel-Morgenstern, Milana, Onn, Itay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586844/
https://www.ncbi.nlm.nih.gov/pubmed/31222142
http://dx.doi.org/10.1038/s41598-019-45263-3
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author Boginya, Alexandra
Detroja, Rajesh
Matityahu, Avi
Frenkel-Morgenstern, Milana
Onn, Itay
author_facet Boginya, Alexandra
Detroja, Rajesh
Matityahu, Avi
Frenkel-Morgenstern, Milana
Onn, Itay
author_sort Boginya, Alexandra
collection PubMed
description Chd1 is a chromatin remodeler that is involved in nucleosome positioning and transcription. Deletion of CHD1 is a frequent event in prostate cancer. The Structural Maintenance of Chromosome (SMC) complex cohesin mediates long-range chromatin interactions and is involved in maintaining genome stability. We provide new evidence that Chd1 is a regulator of cohesin. In the yeast S. cerevisiae, Chd1 is not essential for viability. We show that deletion of the gene leads to a defect in sister chromatid cohesion and in chromosome morphology. Chl1 is a non-essential DNA helicase that has been shown to regulate cohesin loading. Surprisingly, co-deletion of CHD1 and CHL1 results in an additive cohesion defect but partial suppression of the chromosome structure phenotype. We found that the cohesin regulator Pds5 is overexpressed when Chd1 and Chl1 are deleted. However, Pds5 expression is reduced to wild type levels when both genes are deleted. Finally, we show a correlation in the expression of CHD1 and cohesin genes in prostate cancer patients. Furthermore, we show that overexpression of cohesin subunits is correlated with the aggressiveness of the tumor. The biological roles of the interplay between Chd1, Chl1 and SMCs are discussed.
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spelling pubmed-65868442019-06-27 The chromatin remodeler Chd1 regulates cohesin in budding yeast and humans Boginya, Alexandra Detroja, Rajesh Matityahu, Avi Frenkel-Morgenstern, Milana Onn, Itay Sci Rep Article Chd1 is a chromatin remodeler that is involved in nucleosome positioning and transcription. Deletion of CHD1 is a frequent event in prostate cancer. The Structural Maintenance of Chromosome (SMC) complex cohesin mediates long-range chromatin interactions and is involved in maintaining genome stability. We provide new evidence that Chd1 is a regulator of cohesin. In the yeast S. cerevisiae, Chd1 is not essential for viability. We show that deletion of the gene leads to a defect in sister chromatid cohesion and in chromosome morphology. Chl1 is a non-essential DNA helicase that has been shown to regulate cohesin loading. Surprisingly, co-deletion of CHD1 and CHL1 results in an additive cohesion defect but partial suppression of the chromosome structure phenotype. We found that the cohesin regulator Pds5 is overexpressed when Chd1 and Chl1 are deleted. However, Pds5 expression is reduced to wild type levels when both genes are deleted. Finally, we show a correlation in the expression of CHD1 and cohesin genes in prostate cancer patients. Furthermore, we show that overexpression of cohesin subunits is correlated with the aggressiveness of the tumor. The biological roles of the interplay between Chd1, Chl1 and SMCs are discussed. Nature Publishing Group UK 2019-06-20 /pmc/articles/PMC6586844/ /pubmed/31222142 http://dx.doi.org/10.1038/s41598-019-45263-3 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Boginya, Alexandra
Detroja, Rajesh
Matityahu, Avi
Frenkel-Morgenstern, Milana
Onn, Itay
The chromatin remodeler Chd1 regulates cohesin in budding yeast and humans
title The chromatin remodeler Chd1 regulates cohesin in budding yeast and humans
title_full The chromatin remodeler Chd1 regulates cohesin in budding yeast and humans
title_fullStr The chromatin remodeler Chd1 regulates cohesin in budding yeast and humans
title_full_unstemmed The chromatin remodeler Chd1 regulates cohesin in budding yeast and humans
title_short The chromatin remodeler Chd1 regulates cohesin in budding yeast and humans
title_sort chromatin remodeler chd1 regulates cohesin in budding yeast and humans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6586844/
https://www.ncbi.nlm.nih.gov/pubmed/31222142
http://dx.doi.org/10.1038/s41598-019-45263-3
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