Cathepsin L Causes Proteolytic Cleavage of Chinese‐Hamster‐Ovary Cell Expressed Proteins During Processing and Storage: Identification, Characterization, and Mitigation

A stochastic approach of copurification of the protease Cathepsin L that results in product fragmentation during purification processing and storage is presented. Cathepsin L was identified using mass spectroscopy, characterization of proteolytic activity, and comparison with fragmentation patterns...

Descripción completa

Detalles Bibliográficos
Autores principales: Luo, Haibin, Tie, Liu, Cao, Mingyan, Hunter, Alan K., Pabst, Timothy M., Du, Jiali, Field, Raymond, Li, Yuling, Wang, William K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2018
Materias:
RESEARCH ARTICLES
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587562/
https://www.ncbi.nlm.nih.gov/pubmed/30320962
http://dx.doi.org/10.1002/btpr.2732

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587562/
https://www.ncbi.nlm.nih.gov/pubmed/30320962
http://dx.doi.org/10.1002/btpr.2732

Ejemplares similares