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Cathepsin L Causes Proteolytic Cleavage of Chinese‐Hamster‐Ovary Cell Expressed Proteins During Processing and Storage: Identification, Characterization, and Mitigation
A stochastic approach of copurification of the protease Cathepsin L that results in product fragmentation during purification processing and storage is presented. Cathepsin L was identified using mass spectroscopy, characterization of proteolytic activity, and comparison with fragmentation patterns...
Autores principales: | Luo, Haibin, Tie, Liu, Cao, Mingyan, Hunter, Alan K., Pabst, Timothy M., Du, Jiali, Field, Raymond, Li, Yuling, Wang, William K. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587562/ https://www.ncbi.nlm.nih.gov/pubmed/30320962 http://dx.doi.org/10.1002/btpr.2732 |
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