A fast and tuneable auxin‐inducible degron for depletion of target proteins in budding yeast

The auxin‐inducible degron (AID) is a useful technique to rapidly deplete proteins of interest in nonplant eukaryotes. Depletion is achieved by addition of the plant hormone auxin to the cell culture, which allows the auxin‐binding receptor, TIR1, to target the AID‐tagged protein for degradation by...

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Detalles Bibliográficos
Autores principales: Mendoza‐Ochoa, Gonzalo I., Barrass, J. David, Terlouw, Barbara R., Maudlin, Isabella E., de Lucas, Susana, Sani, Emanuela, Aslanzadeh, Vahid, Reid, Jane A.E., Beggs, Jean D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2018
Materias:
Special Issue Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587778/
https://www.ncbi.nlm.nih.gov/pubmed/30375036
http://dx.doi.org/10.1002/yea.3362

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587778/
https://www.ncbi.nlm.nih.gov/pubmed/30375036
http://dx.doi.org/10.1002/yea.3362

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