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A fast and tuneable auxin‐inducible degron for depletion of target proteins in budding yeast
The auxin‐inducible degron (AID) is a useful technique to rapidly deplete proteins of interest in nonplant eukaryotes. Depletion is achieved by addition of the plant hormone auxin to the cell culture, which allows the auxin‐binding receptor, TIR1, to target the AID‐tagged protein for degradation by...
Autores principales: | Mendoza‐Ochoa, Gonzalo I., Barrass, J. David, Terlouw, Barbara R., Maudlin, Isabella E., de Lucas, Susana, Sani, Emanuela, Aslanzadeh, Vahid, Reid, Jane A.E., Beggs, Jean D. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6587778/ https://www.ncbi.nlm.nih.gov/pubmed/30375036 http://dx.doi.org/10.1002/yea.3362 |
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