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Probing the active fraction of soil microbiomes using BONCAT-FACS

The ability to link soil microbial diversity to soil processes requires technologies that differentiate active microbes from extracellular DNA and dormant cells. Here, we use BONCAT (bioorthogonal non-canonical amino acid tagging) to measure translationally active cells in soils. We compare the acti...

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Detalles Bibliográficos
Autores principales: Couradeau, Estelle, Sasse, Joelle, Goudeau, Danielle, Nath, Nandita, Hazen, Terry C., Bowen, Ben P., Chakraborty, Romy, Malmstrom, Rex R., Northen, Trent R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591230/
https://www.ncbi.nlm.nih.gov/pubmed/31235780
http://dx.doi.org/10.1038/s41467-019-10542-0