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Probing the active fraction of soil microbiomes using BONCAT-FACS
The ability to link soil microbial diversity to soil processes requires technologies that differentiate active microbes from extracellular DNA and dormant cells. Here, we use BONCAT (bioorthogonal non-canonical amino acid tagging) to measure translationally active cells in soils. We compare the acti...
Autores principales: | Couradeau, Estelle, Sasse, Joelle, Goudeau, Danielle, Nath, Nandita, Hazen, Terry C., Bowen, Ben P., Chakraborty, Romy, Malmstrom, Rex R., Northen, Trent R. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591230/ https://www.ncbi.nlm.nih.gov/pubmed/31235780 http://dx.doi.org/10.1038/s41467-019-10542-0 |
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