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Stable and transient transformation, and a promoter assay in the selective lignin-degrading fungus, Ceriporiopsis subvermispora

A genetic transformation system was developed for the selective white rot basidiomycete Ceriporiopsis subvermispora using a modified protocol with polyethylene glycol and CaCl(2) treatment of the protoplasts and plasmids harboring recombinant hygromycin phosphotransferase (hph) driven by a homologou...

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Detalles Bibliográficos
Autores principales: Honda, Yoichi, Tanigawa, Eiji, Tsukihara, Takahisa, Nguyen, Dong Xuan, Kawabe, Harunori, Sakatoku, Naofumi, Watari, Junko, Sato, Hideaki, Yano, Shigekazu, Tachiki, Takashi, Irie, Toshikazu, Watanabe, Takahito, Watanabe, Takashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6591348/
https://www.ncbi.nlm.nih.gov/pubmed/31236750
http://dx.doi.org/10.1186/s13568-019-0818-1