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Targeting XIST induced apoptosis of human osteosarcoma cells by activation of NF-kB/PUMA signal

The long noncoding RNA X-inactive specific transcript (XIST) plays vital roles in tumor progression. However, the underlying mechanisms remain unclear. This study investigated the effects and mechanisms of targeting XIST on osteosarcoma (OS) cells in vitro and in vivo. We used shRNA to knockdown XIS...

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Autores principales: Gao, Weiliang, Gao, Jisheng, Chen, Longying, Ren, Yande, Ma, Jinfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6592364/
https://www.ncbi.nlm.nih.gov/pubmed/31189404
http://dx.doi.org/10.1080/21655979.2019.1631104
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author Gao, Weiliang
Gao, Jisheng
Chen, Longying
Ren, Yande
Ma, Jinfeng
author_facet Gao, Weiliang
Gao, Jisheng
Chen, Longying
Ren, Yande
Ma, Jinfeng
author_sort Gao, Weiliang
collection PubMed
description The long noncoding RNA X-inactive specific transcript (XIST) plays vital roles in tumor progression. However, the underlying mechanisms remain unclear. This study investigated the effects and mechanisms of targeting XIST on osteosarcoma (OS) cells in vitro and in vivo. We used shRNA to knockdown XIST to evaluate cell growth and apoptosis in U2OS cells in vitro and xenograft formation in vivo. An observed relationship between XIST and the p53 upregulated modulator of apoptosis (PUMA) and nuclear factor-kappa B (NF-kB) pathway was further explored by using small interfering RNA (siRNA). Our results showed that suppression of XIST by short hairpin RNA (shRNA) impeded U2OS cell growth, induced apoptosis and lessened OS xenograft tumor growth. Targeting XIST increased NF-kB-dependent PUMA upregulation in U2OS cells. Upregulation of PUMA is correlated with suppression of XIST-induced apoptosis in U2OS cells. Therefore, inhibition of XIST could promote U2OS cell death via activation of NF-kB/PUMA pathways.
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spelling pubmed-65923642020-06-16 Targeting XIST induced apoptosis of human osteosarcoma cells by activation of NF-kB/PUMA signal Gao, Weiliang Gao, Jisheng Chen, Longying Ren, Yande Ma, Jinfeng Bioengineered Research Paper The long noncoding RNA X-inactive specific transcript (XIST) plays vital roles in tumor progression. However, the underlying mechanisms remain unclear. This study investigated the effects and mechanisms of targeting XIST on osteosarcoma (OS) cells in vitro and in vivo. We used shRNA to knockdown XIST to evaluate cell growth and apoptosis in U2OS cells in vitro and xenograft formation in vivo. An observed relationship between XIST and the p53 upregulated modulator of apoptosis (PUMA) and nuclear factor-kappa B (NF-kB) pathway was further explored by using small interfering RNA (siRNA). Our results showed that suppression of XIST by short hairpin RNA (shRNA) impeded U2OS cell growth, induced apoptosis and lessened OS xenograft tumor growth. Targeting XIST increased NF-kB-dependent PUMA upregulation in U2OS cells. Upregulation of PUMA is correlated with suppression of XIST-induced apoptosis in U2OS cells. Therefore, inhibition of XIST could promote U2OS cell death via activation of NF-kB/PUMA pathways. Taylor & Francis 2019-06-16 /pmc/articles/PMC6592364/ /pubmed/31189404 http://dx.doi.org/10.1080/21655979.2019.1631104 Text en © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Gao, Weiliang
Gao, Jisheng
Chen, Longying
Ren, Yande
Ma, Jinfeng
Targeting XIST induced apoptosis of human osteosarcoma cells by activation of NF-kB/PUMA signal
title Targeting XIST induced apoptosis of human osteosarcoma cells by activation of NF-kB/PUMA signal
title_full Targeting XIST induced apoptosis of human osteosarcoma cells by activation of NF-kB/PUMA signal
title_fullStr Targeting XIST induced apoptosis of human osteosarcoma cells by activation of NF-kB/PUMA signal
title_full_unstemmed Targeting XIST induced apoptosis of human osteosarcoma cells by activation of NF-kB/PUMA signal
title_short Targeting XIST induced apoptosis of human osteosarcoma cells by activation of NF-kB/PUMA signal
title_sort targeting xist induced apoptosis of human osteosarcoma cells by activation of nf-kb/puma signal
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6592364/
https://www.ncbi.nlm.nih.gov/pubmed/31189404
http://dx.doi.org/10.1080/21655979.2019.1631104
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