In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens

The genetic interactions influencing metastatic potential have been challenging to investigate systematically. Here we developed MCAP (massively parallel CRISPR-Cpf1/Cas12a crRNA array profiling), an approach for combinatorial interrogation of double knockouts in vivo. We designed an MCAP library of...

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Detalles Bibliográficos
Autores principales: Chow, Ryan D., Wang, Guangchuan, Ye, Lupeng, Codina, Adan, Kim, Hyunu Ray, Shen, Li, Dong, Matthew B., Errami, Youssef, Chen, Sidi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6592845/
https://www.ncbi.nlm.nih.gov/pubmed/30962622
http://dx.doi.org/10.1038/s41592-019-0371-5
Descripción
Sumario:The genetic interactions influencing metastatic potential have been challenging to investigate systematically. Here we developed MCAP (massively parallel CRISPR-Cpf1/Cas12a crRNA array profiling), an approach for combinatorial interrogation of double knockouts in vivo. We designed an MCAP library of 11,934 arrays targeting 325 pairwise combinations of genes implicated in metastasis. By assessing the metastatic potential of the double knockouts in mice, we unveiled a quantitative landscape of genetic interactions driving metastasis.

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