In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens

The genetic interactions influencing metastatic potential have been challenging to investigate systematically. Here we developed MCAP (massively parallel CRISPR-Cpf1/Cas12a crRNA array profiling), an approach for combinatorial interrogation of double knockouts in vivo. We designed an MCAP library of...

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Detalles Bibliográficos
Autores principales: Chow, Ryan D., Wang, Guangchuan, Ye, Lupeng, Codina, Adan, Kim, Hyunu Ray, Shen, Li, Dong, Matthew B., Errami, Youssef, Chen, Sidi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6592845/
https://www.ncbi.nlm.nih.gov/pubmed/30962622
http://dx.doi.org/10.1038/s41592-019-0371-5
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author Chow, Ryan D.
Wang, Guangchuan
Ye, Lupeng
Codina, Adan
Kim, Hyunu Ray
Shen, Li
Dong, Matthew B.
Errami, Youssef
Chen, Sidi
author_facet Chow, Ryan D.
Wang, Guangchuan
Ye, Lupeng
Codina, Adan
Kim, Hyunu Ray
Shen, Li
Dong, Matthew B.
Errami, Youssef
Chen, Sidi
author_sort Chow, Ryan D.
collection PubMed
description The genetic interactions influencing metastatic potential have been challenging to investigate systematically. Here we developed MCAP (massively parallel CRISPR-Cpf1/Cas12a crRNA array profiling), an approach for combinatorial interrogation of double knockouts in vivo. We designed an MCAP library of 11,934 arrays targeting 325 pairwise combinations of genes implicated in metastasis. By assessing the metastatic potential of the double knockouts in mice, we unveiled a quantitative landscape of genetic interactions driving metastasis.
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spelling pubmed-65928452019-10-08 In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens Chow, Ryan D. Wang, Guangchuan Ye, Lupeng Codina, Adan Kim, Hyunu Ray Shen, Li Dong, Matthew B. Errami, Youssef Chen, Sidi Nat Methods Article The genetic interactions influencing metastatic potential have been challenging to investigate systematically. Here we developed MCAP (massively parallel CRISPR-Cpf1/Cas12a crRNA array profiling), an approach for combinatorial interrogation of double knockouts in vivo. We designed an MCAP library of 11,934 arrays targeting 325 pairwise combinations of genes implicated in metastasis. By assessing the metastatic potential of the double knockouts in mice, we unveiled a quantitative landscape of genetic interactions driving metastasis. 2019-04-08 2019-05 /pmc/articles/PMC6592845/ /pubmed/30962622 http://dx.doi.org/10.1038/s41592-019-0371-5 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Chow, Ryan D.
Wang, Guangchuan
Ye, Lupeng
Codina, Adan
Kim, Hyunu Ray
Shen, Li
Dong, Matthew B.
Errami, Youssef
Chen, Sidi
In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens
title In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens
title_full In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens
title_fullStr In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens
title_full_unstemmed In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens
title_short In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens
title_sort in vivo profiling of metastatic double knockouts through crispr-cpf1 screens
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6592845/
https://www.ncbi.nlm.nih.gov/pubmed/30962622
http://dx.doi.org/10.1038/s41592-019-0371-5
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