RNA‐guided endonuclease – in situ labelling (RGEN‐ISL): a fast CRISPR/Cas9‐based method to label genomic sequences in various species

Visualising the spatio‐temporal organisation of the genome will improve our understanding of how chromatin structure and function are intertwined. We developed a tool to visualise defined genomic sequences in fixed nuclei and chromosomes based on a two‐part guide RNA with a recombinant Cas9 endonucl...

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Detalles Bibliográficos
Autores principales: Ishii, Takayoshi, Schubert, Veit, Khosravi, Solmaz, Dreissig, Steven, Metje‐Sprink, Janina, Sprink, Thorben, Fuchs, Jörg, Meister, Armin, Houben, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6593734/
https://www.ncbi.nlm.nih.gov/pubmed/30847946
http://dx.doi.org/10.1111/nph.15720

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6593734/
https://www.ncbi.nlm.nih.gov/pubmed/30847946
http://dx.doi.org/10.1111/nph.15720

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