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Ionisation bias undermines the use of matrix‐assisted laser desorption/ionisation for estimating peptide deamidation: Synthetic peptide studies demonstrate electrospray ionisation gives more reliable response ratios
RATIONALE: Although mass spectrometry (MS) is routinely used to determine deamination in peptide mixtures, the effects of the choice of ionisation source have not yet been investigated. In particular, matrix‐assisted laser desorption/ionisation (MALDI) has become a popular tool with which to measure...
Autores principales: | Simpson, Joanna P., Fascione, Martin, Bergström, Ed, Wilson, Julie, Collins, Matthew J., Penkman, Kirsty E.H., Thomas‐Oates, Jane |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594239/ https://www.ncbi.nlm.nih.gov/pubmed/30908787 http://dx.doi.org/10.1002/rcm.8441 |
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