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Dissociation of membrane–chromatin contacts is required for proper chromosome segregation in mitosis
The nuclear envelope (NE) aids in organizing the interphase genome by tethering chromatin to the nuclear periphery. During mitotic entry, NE–chromatin contacts are broken. Here, we report on the consequences of impaired NE removal from chromatin for cell division of human cells. Using a membrane–chr...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594442/ https://www.ncbi.nlm.nih.gov/pubmed/30586323 http://dx.doi.org/10.1091/mbc.E18-10-0609 |
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author | Champion, Lysie Pawar, Sumit Luithle, Naemi Ungricht, Rosemarie Kutay, Ulrike |
author_facet | Champion, Lysie Pawar, Sumit Luithle, Naemi Ungricht, Rosemarie Kutay, Ulrike |
author_sort | Champion, Lysie |
collection | PubMed |
description | The nuclear envelope (NE) aids in organizing the interphase genome by tethering chromatin to the nuclear periphery. During mitotic entry, NE–chromatin contacts are broken. Here, we report on the consequences of impaired NE removal from chromatin for cell division of human cells. Using a membrane–chromatin tether that cannot be dissociated when cells enter mitosis, we show that a failure in breaking membrane–chromatin interactions impairs mitotic chromatin organization, chromosome segregation and cytokinesis, and induces an aberrant NE morphology in postmitotic cells. In contrast, chromosome segregation and cell division proceed successfully when membrane attachment to chromatin is induced during metaphase, after chromosomes have been singularized and aligned at the metaphase plate. These results indicate that the separation of membranes and chromatin is critical during prometaphase to allow for proper chromosome compaction and segregation. We propose that one cause of these defects is the multivalency of membrane–chromatin interactions. |
format | Online Article Text |
id | pubmed-6594442 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-65944422019-07-05 Dissociation of membrane–chromatin contacts is required for proper chromosome segregation in mitosis Champion, Lysie Pawar, Sumit Luithle, Naemi Ungricht, Rosemarie Kutay, Ulrike Mol Biol Cell Articles The nuclear envelope (NE) aids in organizing the interphase genome by tethering chromatin to the nuclear periphery. During mitotic entry, NE–chromatin contacts are broken. Here, we report on the consequences of impaired NE removal from chromatin for cell division of human cells. Using a membrane–chromatin tether that cannot be dissociated when cells enter mitosis, we show that a failure in breaking membrane–chromatin interactions impairs mitotic chromatin organization, chromosome segregation and cytokinesis, and induces an aberrant NE morphology in postmitotic cells. In contrast, chromosome segregation and cell division proceed successfully when membrane attachment to chromatin is induced during metaphase, after chromosomes have been singularized and aligned at the metaphase plate. These results indicate that the separation of membranes and chromatin is critical during prometaphase to allow for proper chromosome compaction and segregation. We propose that one cause of these defects is the multivalency of membrane–chromatin interactions. The American Society for Cell Biology 2019-02-15 /pmc/articles/PMC6594442/ /pubmed/30586323 http://dx.doi.org/10.1091/mbc.E18-10-0609 Text en © 2019 Champion et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. http://creativecommons.org/licenses/by-nc-sa/3.0 This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License. |
spellingShingle | Articles Champion, Lysie Pawar, Sumit Luithle, Naemi Ungricht, Rosemarie Kutay, Ulrike Dissociation of membrane–chromatin contacts is required for proper chromosome segregation in mitosis |
title | Dissociation of membrane–chromatin contacts is required for proper chromosome segregation in mitosis |
title_full | Dissociation of membrane–chromatin contacts is required for proper chromosome segregation in mitosis |
title_fullStr | Dissociation of membrane–chromatin contacts is required for proper chromosome segregation in mitosis |
title_full_unstemmed | Dissociation of membrane–chromatin contacts is required for proper chromosome segregation in mitosis |
title_short | Dissociation of membrane–chromatin contacts is required for proper chromosome segregation in mitosis |
title_sort | dissociation of membrane–chromatin contacts is required for proper chromosome segregation in mitosis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6594442/ https://www.ncbi.nlm.nih.gov/pubmed/30586323 http://dx.doi.org/10.1091/mbc.E18-10-0609 |
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