Catalyst-free Click PEGylation reveals substantial mitochondrial ATP synthase sub-unit alpha oxidation before and after fertilisation

Using non-reducing Western blotting to assess protein thiol redox state is challenging because most reduced and oxidised forms migrate at the same molecular weight and are, therefore, indistinguishable. While copper catalysed Click chemistry can be used to ligate a polyethylene glycol (PEG) moiety t...

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Detalles Bibliográficos
Autores principales: Cobley, James N., Noble, Anna, Jimenez-Fernandez, Eduardo, Valdivia Moya, Manuel-Thomas, Guille, Matthew, Husi, Holger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6597785/
https://www.ncbi.nlm.nih.gov/pubmed/31234016
http://dx.doi.org/10.1016/j.redox.2019.101258

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6597785/
https://www.ncbi.nlm.nih.gov/pubmed/31234016
http://dx.doi.org/10.1016/j.redox.2019.101258

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