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Directed evolution of a genetically encoded immobilized lipase for the efficient production of biodiesel from waste cooking oil

BACKGROUND: We have recently developed a one-step, genetically encoded immobilization approach based on fusion of a target enzyme to the self-crystallizing protein Cry3Aa, followed by direct production and isolation of the fusion crystals from Bacillus thuringiensis. Using this approach, Bacillus su...

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Detalles Bibliográficos
Autores principales: Heater, Bradley S., Chan, Wai Shan, Lee, Marianne M., Chan, Michael K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6598307/
https://www.ncbi.nlm.nih.gov/pubmed/31297153
http://dx.doi.org/10.1186/s13068-019-1509-5