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Directed evolution of a genetically encoded immobilized lipase for the efficient production of biodiesel from waste cooking oil
BACKGROUND: We have recently developed a one-step, genetically encoded immobilization approach based on fusion of a target enzyme to the self-crystallizing protein Cry3Aa, followed by direct production and isolation of the fusion crystals from Bacillus thuringiensis. Using this approach, Bacillus su...
Autores principales: | Heater, Bradley S., Chan, Wai Shan, Lee, Marianne M., Chan, Michael K. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6598307/ https://www.ncbi.nlm.nih.gov/pubmed/31297153 http://dx.doi.org/10.1186/s13068-019-1509-5 |
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