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Improved CUT&RUN chromatin profiling tools

Previously, we described a novel alternative to chromatin immunoprecipitation, CUT&RUN, in which unfixed permeabilized cells are incubated with antibody, followed by binding of a protein A-Micrococcal Nuclease (pA/MNase) fusion protein (Skene and Henikoff, 2017). Here we introduce three enhancem...

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Detalles Bibliográficos
Autores principales: Meers, Michael P, Bryson, Terri D, Henikoff, Jorja G, Henikoff, Steven
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6598765/
https://www.ncbi.nlm.nih.gov/pubmed/31232687
http://dx.doi.org/10.7554/eLife.46314