Generating viable mice with heritable embryonically lethal mutations using the CRISPR-Cas9 system in two-cell embryos

A substantial number of mouse genes, about 25%, are embryonically lethal when knocked out. Using current genetic tools, such as the CRISPR-Cas9 system, it is difficult—or even impossible—to produce viable mice with heritable embryonically lethal mutations. Here, we establish a one-step method for mi...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Yi, Zhang, Jing, Peng, Boya, Tian, Dan, Zhang, Dong, Li, Yang, Feng, Xiaoyu, Liu, Jinghao, Li, Jun, Zhang, Teng, Liu, Xiaoyong, Lu, Jing, Chen, Baian, Wang, Songlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6599060/
https://www.ncbi.nlm.nih.gov/pubmed/31253768
http://dx.doi.org/10.1038/s41467-019-10748-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6599060/
https://www.ncbi.nlm.nih.gov/pubmed/31253768
http://dx.doi.org/10.1038/s41467-019-10748-2

Ejemplares similares