Robotic assisted generation of 2′-deoxy-2′-fluoro-modifed RNA aptamers – High performance enabling strategies in aptamer selection

Aptamer selection is a laborious procedure, requiring expertise and significant resources. These characteristics limit the accessibility of researchers to these molecular tools. We describe a selection procedure, making use of a robotic system that allows the fully automated selection of RNA and 2′d...

Descripción completa

Detalles Bibliográficos
Autores principales: Breuers, Stefan, Bryant, Laura Lledo, Legen, Tjasa, Mayer, Günter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academic Press 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6599171/
https://www.ncbi.nlm.nih.gov/pubmed/31152781
http://dx.doi.org/10.1016/j.ymeth.2019.05.022
_version_ 1783430907090173952
author Breuers, Stefan
Bryant, Laura Lledo
Legen, Tjasa
Mayer, Günter
author_facet Breuers, Stefan
Bryant, Laura Lledo
Legen, Tjasa
Mayer, Günter
author_sort Breuers, Stefan
collection PubMed
description Aptamer selection is a laborious procedure, requiring expertise and significant resources. These characteristics limit the accessibility of researchers to these molecular tools. We describe a selection procedure, making use of a robotic system that allows the fully automated selection of RNA and 2′deoxy-2′-fluoro pyrimidine RNA aptamers. The platform offers a rapid access to aptamers for basic research and development, therefore opening the path to aptamer-based systemic analysis of proteomes in biological settings.
format Online
Article
Text
id pubmed-6599171
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Academic Press
record_format MEDLINE/PubMed
spelling pubmed-65991712019-07-11 Robotic assisted generation of 2′-deoxy-2′-fluoro-modifed RNA aptamers – High performance enabling strategies in aptamer selection Breuers, Stefan Bryant, Laura Lledo Legen, Tjasa Mayer, Günter Methods Article Aptamer selection is a laborious procedure, requiring expertise and significant resources. These characteristics limit the accessibility of researchers to these molecular tools. We describe a selection procedure, making use of a robotic system that allows the fully automated selection of RNA and 2′deoxy-2′-fluoro pyrimidine RNA aptamers. The platform offers a rapid access to aptamers for basic research and development, therefore opening the path to aptamer-based systemic analysis of proteomes in biological settings. Academic Press 2019-05-15 /pmc/articles/PMC6599171/ /pubmed/31152781 http://dx.doi.org/10.1016/j.ymeth.2019.05.022 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Breuers, Stefan
Bryant, Laura Lledo
Legen, Tjasa
Mayer, Günter
Robotic assisted generation of 2′-deoxy-2′-fluoro-modifed RNA aptamers – High performance enabling strategies in aptamer selection
title Robotic assisted generation of 2′-deoxy-2′-fluoro-modifed RNA aptamers – High performance enabling strategies in aptamer selection
title_full Robotic assisted generation of 2′-deoxy-2′-fluoro-modifed RNA aptamers – High performance enabling strategies in aptamer selection
title_fullStr Robotic assisted generation of 2′-deoxy-2′-fluoro-modifed RNA aptamers – High performance enabling strategies in aptamer selection
title_full_unstemmed Robotic assisted generation of 2′-deoxy-2′-fluoro-modifed RNA aptamers – High performance enabling strategies in aptamer selection
title_short Robotic assisted generation of 2′-deoxy-2′-fluoro-modifed RNA aptamers – High performance enabling strategies in aptamer selection
title_sort robotic assisted generation of 2′-deoxy-2′-fluoro-modifed rna aptamers – high performance enabling strategies in aptamer selection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6599171/
https://www.ncbi.nlm.nih.gov/pubmed/31152781
http://dx.doi.org/10.1016/j.ymeth.2019.05.022
work_keys_str_mv AT breuersstefan roboticassistedgenerationof2deoxy2fluoromodifedrnaaptamershighperformanceenablingstrategiesinaptamerselection
AT bryantlauralledo roboticassistedgenerationof2deoxy2fluoromodifedrnaaptamershighperformanceenablingstrategiesinaptamerselection
AT legentjasa roboticassistedgenerationof2deoxy2fluoromodifedrnaaptamershighperformanceenablingstrategiesinaptamerselection
AT mayergunter roboticassistedgenerationof2deoxy2fluoromodifedrnaaptamershighperformanceenablingstrategiesinaptamerselection

Ejemplares similares