Time-Gated Luminescent In Situ Hybridization (LISH): Highly Sensitive Detection of Pathogenic Staphylococcus aureus

We describe simple direct conjugation of a single TEGylated Europium chelate to DNA that binds to intracellular rRNA and is then detected using a homogeneous luminescent in situ hybridisation (LISH) technique. As a proof-of-principle, Staphylococcus aureus (S. aureus) was selected as a model for our...

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Detalles Bibliográficos
Autores principales: Sayyadi, Nima, Connally, Russell E., Lawson, Thomas S., Yuan, Jingli, Packer, Nicolle H., Piper, James A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600140/
https://www.ncbi.nlm.nih.gov/pubmed/31159269
http://dx.doi.org/10.3390/molecules24112083

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600140/
https://www.ncbi.nlm.nih.gov/pubmed/31159269
http://dx.doi.org/10.3390/molecules24112083

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