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Time-Gated Luminescent In Situ Hybridization (LISH): Highly Sensitive Detection of Pathogenic Staphylococcus aureus
We describe simple direct conjugation of a single TEGylated Europium chelate to DNA that binds to intracellular rRNA and is then detected using a homogeneous luminescent in situ hybridisation (LISH) technique. As a proof-of-principle, Staphylococcus aureus (S. aureus) was selected as a model for our...
Autores principales: | Sayyadi, Nima, Connally, Russell E., Lawson, Thomas S., Yuan, Jingli, Packer, Nicolle H., Piper, James A. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600140/ https://www.ncbi.nlm.nih.gov/pubmed/31159269 http://dx.doi.org/10.3390/molecules24112083 |
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