Cargando…
Multiplex Real-Time PCR with HRM for Detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples
Optimization of fermentation processes requires monitoring the species composition of starter cultures and their growth during fermentation. Most starter cultures contain closely related species. Nowadays, high-resolution melting (HRM) analysis is extensively used for multiplex identification of clo...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
University of Zagreb Faculty of Food Technology and Biotechnology
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600297/ https://www.ncbi.nlm.nih.gov/pubmed/31316281 http://dx.doi.org/10.17113/ftb.57.01.19.5983 |
_version_ | 1783431085383745536 |
---|---|
author | Kurbakov, Konstantin A. Konorov, Evgenii A. Minaev, Mikhail Y. Kuznetsova, Oksana A. |
author_facet | Kurbakov, Konstantin A. Konorov, Evgenii A. Minaev, Mikhail Y. Kuznetsova, Oksana A. |
author_sort | Kurbakov, Konstantin A. |
collection | PubMed |
description | Optimization of fermentation processes requires monitoring the species composition of starter cultures and their growth during fermentation. Most starter cultures contain closely related species. Nowadays, high-resolution melting (HRM) analysis is extensively used for multiplex identification of closely related species. In the present paper, we applied real-time polymerase chain reaction (PCR) with HRM analysis for the detection and differentiation of Lactobacillus sakei and L. curvatus. A primer pair was selected for the site of the rpoA gene of Lactobacillus spp. Eleven starter cultures and fifteen fermented sausages with a known bacterial composition were successfully tested using real-time PCR with HRM analysis with the developed primer pair. |
format | Online Article Text |
id | pubmed-6600297 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
record_format | MEDLINE/PubMed |
spelling | pubmed-66002972019-07-17 Multiplex Real-Time PCR with HRM for Detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples Kurbakov, Konstantin A. Konorov, Evgenii A. Minaev, Mikhail Y. Kuznetsova, Oksana A. Food Technol Biotechnol Preliminary Communications Optimization of fermentation processes requires monitoring the species composition of starter cultures and their growth during fermentation. Most starter cultures contain closely related species. Nowadays, high-resolution melting (HRM) analysis is extensively used for multiplex identification of closely related species. In the present paper, we applied real-time polymerase chain reaction (PCR) with HRM analysis for the detection and differentiation of Lactobacillus sakei and L. curvatus. A primer pair was selected for the site of the rpoA gene of Lactobacillus spp. Eleven starter cultures and fifteen fermented sausages with a known bacterial composition were successfully tested using real-time PCR with HRM analysis with the developed primer pair. University of Zagreb Faculty of Food Technology and Biotechnology 2019-03 /pmc/articles/PMC6600297/ /pubmed/31316281 http://dx.doi.org/10.17113/ftb.57.01.19.5983 Text en http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial (CC BY-NC) 4.0 License. |
spellingShingle | Preliminary Communications Kurbakov, Konstantin A. Konorov, Evgenii A. Minaev, Mikhail Y. Kuznetsova, Oksana A. Multiplex Real-Time PCR with HRM for Detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples |
title | Multiplex Real-Time PCR with HRM for Detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples |
title_full | Multiplex Real-Time PCR with HRM for Detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples |
title_fullStr | Multiplex Real-Time PCR with HRM for Detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples |
title_full_unstemmed | Multiplex Real-Time PCR with HRM for Detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples |
title_short | Multiplex Real-Time PCR with HRM for Detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples |
title_sort | multiplex real-time pcr with hrm for detection of lactobacillus sakei and lactobacillus curvatus in food samples |
topic | Preliminary Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6600297/ https://www.ncbi.nlm.nih.gov/pubmed/31316281 http://dx.doi.org/10.17113/ftb.57.01.19.5983 |
work_keys_str_mv | AT kurbakovkonstantina multiplexrealtimepcrwithhrmfordetectionoflactobacillussakeiandlactobacilluscurvatusinfoodsamples AT konorovevgeniia multiplexrealtimepcrwithhrmfordetectionoflactobacillussakeiandlactobacilluscurvatusinfoodsamples AT minaevmikhaily multiplexrealtimepcrwithhrmfordetectionoflactobacillussakeiandlactobacilluscurvatusinfoodsamples AT kuznetsovaoksanaa multiplexrealtimepcrwithhrmfordetectionoflactobacillussakeiandlactobacilluscurvatusinfoodsamples |