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Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery
The Piwi-interacting RNA (piRNA) pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. piRNA biogenesis requires a specialized machinery that converts long single-stranded precursors into small RNAs of ∼25-nucleotides in length. This process involves f...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6601507/ https://www.ncbi.nlm.nih.gov/pubmed/31123065 http://dx.doi.org/10.1101/gad.325662.119 |
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author | Munafò, Marzia Manelli, Vera Falconio, Federica A. Sawle, Ashley Kneuss, Emma Eastwood, Evelyn L. Seah, Jun Wen Eugene Czech, Benjamin Hannon, Gregory J. |
author_facet | Munafò, Marzia Manelli, Vera Falconio, Federica A. Sawle, Ashley Kneuss, Emma Eastwood, Evelyn L. Seah, Jun Wen Eugene Czech, Benjamin Hannon, Gregory J. |
author_sort | Munafò, Marzia |
collection | PubMed |
description | The Piwi-interacting RNA (piRNA) pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. piRNA biogenesis requires a specialized machinery that converts long single-stranded precursors into small RNAs of ∼25-nucleotides in length. This process involves factors that operate in two different subcellular compartments: the nuage/Yb body and mitochondria. How these two sites communicate to achieve accurate substrate selection and efficient processing remains unclear. Here, we investigate a previously uncharacterized piRNA biogenesis factor, Daedalus (Daed), that is located on the outer mitochondrial membrane. Daed is essential for Zucchini-mediated piRNA production and the correct localization of the indispensable piRNA biogenesis factor Armitage (Armi). We found that Gasz and Daed interact with each other and likely provide a mitochondrial “anchoring platform” to ensure that Armi is held in place, proximal to Zucchini, during piRNA processing. Our data suggest that Armi initially identifies piRNA precursors in nuage/Yb bodies in a manner that depends on Piwi and then moves to mitochondria to present precursors to the mitochondrial biogenesis machinery. These results represent a significant step in understanding a critical aspect of transposon silencing; namely, how RNAs are chosen to instruct the piRNA machinery in the nature of its silencing targets. |
format | Online Article Text |
id | pubmed-6601507 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Cold Spring Harbor Laboratory Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-66015072019-07-18 Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery Munafò, Marzia Manelli, Vera Falconio, Federica A. Sawle, Ashley Kneuss, Emma Eastwood, Evelyn L. Seah, Jun Wen Eugene Czech, Benjamin Hannon, Gregory J. Genes Dev Research Paper The Piwi-interacting RNA (piRNA) pathway is a small RNA-based immune system that silences mobile genetic elements in animal germlines. piRNA biogenesis requires a specialized machinery that converts long single-stranded precursors into small RNAs of ∼25-nucleotides in length. This process involves factors that operate in two different subcellular compartments: the nuage/Yb body and mitochondria. How these two sites communicate to achieve accurate substrate selection and efficient processing remains unclear. Here, we investigate a previously uncharacterized piRNA biogenesis factor, Daedalus (Daed), that is located on the outer mitochondrial membrane. Daed is essential for Zucchini-mediated piRNA production and the correct localization of the indispensable piRNA biogenesis factor Armitage (Armi). We found that Gasz and Daed interact with each other and likely provide a mitochondrial “anchoring platform” to ensure that Armi is held in place, proximal to Zucchini, during piRNA processing. Our data suggest that Armi initially identifies piRNA precursors in nuage/Yb bodies in a manner that depends on Piwi and then moves to mitochondria to present precursors to the mitochondrial biogenesis machinery. These results represent a significant step in understanding a critical aspect of transposon silencing; namely, how RNAs are chosen to instruct the piRNA machinery in the nature of its silencing targets. Cold Spring Harbor Laboratory Press 2019-07-01 /pmc/articles/PMC6601507/ /pubmed/31123065 http://dx.doi.org/10.1101/gad.325662.119 Text en © 2019 Munafò et al.; Published by Cold Spring Harbor Laboratory Press http://creativecommons.org/licenses/by-nc/4.0/ This article, published in Genes & Development, is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/. |
spellingShingle | Research Paper Munafò, Marzia Manelli, Vera Falconio, Federica A. Sawle, Ashley Kneuss, Emma Eastwood, Evelyn L. Seah, Jun Wen Eugene Czech, Benjamin Hannon, Gregory J. Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery |
title | Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery |
title_full | Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery |
title_fullStr | Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery |
title_full_unstemmed | Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery |
title_short | Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery |
title_sort | daedalus and gasz recruit armitage to mitochondria, bringing pirna precursors to the biogenesis machinery |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6601507/ https://www.ncbi.nlm.nih.gov/pubmed/31123065 http://dx.doi.org/10.1101/gad.325662.119 |
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