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Human spermatozoa anti-proprotein convertase subtilisin/kexin type 4 synthesis using New Zealand rabbit for novel immunocontraception in males

PURPOSE: Proprotein convertase subtilisin/kexin type 4 (PCSK4, a 54-kDa protease) is expressed in the plasma membrane of the acrosome in human spermatozoa. It plays a critical role in penetrating the zona pellucida. Synthesis of human anti-PCSK4 might be important for novel male immunocontraception....

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Autores principales: Dahril, Aulanni'am, Keumala, Vivi, Mustafa, Akhmad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Urological Association 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6607073/
https://www.ncbi.nlm.nih.gov/pubmed/31294140
http://dx.doi.org/10.4111/icu.2019.60.4.303
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author Dahril,
Aulanni'am,
Keumala, Vivi
Mustafa, Akhmad
author_facet Dahril,
Aulanni'am,
Keumala, Vivi
Mustafa, Akhmad
author_sort Dahril,
collection PubMed
description PURPOSE: Proprotein convertase subtilisin/kexin type 4 (PCSK4, a 54-kDa protease) is expressed in the plasma membrane of the acrosome in human spermatozoa. It plays a critical role in penetrating the zona pellucida. Synthesis of human anti-PCSK4 might be important for novel male immunocontraception. MATERIALS AND METHODS: We used semen from adult males as the source of antigen (acrosomal PCSK4). Isolation and antigen characterization were done by immunohistochemistry followed by electrophoresis. Purification of PCSK4 was done by the electroelution method, followed by immunization with an animal model (Oryctolagus cuniculus). Antibody was collected, purified, and tested by using Western blot and dot blot. Antibody in vitro potential testing was performed on human spermatozoa by laser scanning microscopy with rhodamine stain under a light microscope and on rat spermatozoa. RESULTS: Human anti-PCSK4 bound with PCSK4 on the head of human spermatozoa and could interfere with rat spermatozoa activity to penetrate the oocyte. CONCLUSIONS: The result of this study can be used as a basis to develop new immunocontraceptives targeted towards males. This study shows that antibodies from induction of PCSK4 from spermatozoa may hinder fertilization.
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spelling pubmed-66070732019-07-10 Human spermatozoa anti-proprotein convertase subtilisin/kexin type 4 synthesis using New Zealand rabbit for novel immunocontraception in males Dahril, Aulanni'am, Keumala, Vivi Mustafa, Akhmad Investig Clin Urol Innovations in Urology PURPOSE: Proprotein convertase subtilisin/kexin type 4 (PCSK4, a 54-kDa protease) is expressed in the plasma membrane of the acrosome in human spermatozoa. It plays a critical role in penetrating the zona pellucida. Synthesis of human anti-PCSK4 might be important for novel male immunocontraception. MATERIALS AND METHODS: We used semen from adult males as the source of antigen (acrosomal PCSK4). Isolation and antigen characterization were done by immunohistochemistry followed by electrophoresis. Purification of PCSK4 was done by the electroelution method, followed by immunization with an animal model (Oryctolagus cuniculus). Antibody was collected, purified, and tested by using Western blot and dot blot. Antibody in vitro potential testing was performed on human spermatozoa by laser scanning microscopy with rhodamine stain under a light microscope and on rat spermatozoa. RESULTS: Human anti-PCSK4 bound with PCSK4 on the head of human spermatozoa and could interfere with rat spermatozoa activity to penetrate the oocyte. CONCLUSIONS: The result of this study can be used as a basis to develop new immunocontraceptives targeted towards males. This study shows that antibodies from induction of PCSK4 from spermatozoa may hinder fertilization. The Korean Urological Association 2019-07 2019-06-12 /pmc/articles/PMC6607073/ /pubmed/31294140 http://dx.doi.org/10.4111/icu.2019.60.4.303 Text en © The Korean Urological Association, 2019 http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Innovations in Urology
Dahril,
Aulanni'am,
Keumala, Vivi
Mustafa, Akhmad
Human spermatozoa anti-proprotein convertase subtilisin/kexin type 4 synthesis using New Zealand rabbit for novel immunocontraception in males
title Human spermatozoa anti-proprotein convertase subtilisin/kexin type 4 synthesis using New Zealand rabbit for novel immunocontraception in males
title_full Human spermatozoa anti-proprotein convertase subtilisin/kexin type 4 synthesis using New Zealand rabbit for novel immunocontraception in males
title_fullStr Human spermatozoa anti-proprotein convertase subtilisin/kexin type 4 synthesis using New Zealand rabbit for novel immunocontraception in males
title_full_unstemmed Human spermatozoa anti-proprotein convertase subtilisin/kexin type 4 synthesis using New Zealand rabbit for novel immunocontraception in males
title_short Human spermatozoa anti-proprotein convertase subtilisin/kexin type 4 synthesis using New Zealand rabbit for novel immunocontraception in males
title_sort human spermatozoa anti-proprotein convertase subtilisin/kexin type 4 synthesis using new zealand rabbit for novel immunocontraception in males
topic Innovations in Urology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6607073/
https://www.ncbi.nlm.nih.gov/pubmed/31294140
http://dx.doi.org/10.4111/icu.2019.60.4.303
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