PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy

Cryo-electron tomography (cryo-ET) is emerging as a revolutionary method for resolving the structure of macromolecular complexes in situ. However, sample preparation for in situ Cryo-ET is labour-intensive and can require both cryo-lamella preparation through cryo-focused ion beam (FIB) milling and...

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Detalles Bibliográficos
Autores principales: Gorelick, Sergey, Buckley, Genevieve, Gervinskas, Gediminas, Johnson, Travis K, Handley, Ava, Caggiano, Monica Pia, Whisstock, James C, Pocock, Roger, de Marco, Alex
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2019
Materias:
Structural Biology and Molecular Biophysics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609333/
https://www.ncbi.nlm.nih.gov/pubmed/31259689
http://dx.doi.org/10.7554/eLife.45919

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609333/
https://www.ncbi.nlm.nih.gov/pubmed/31259689
http://dx.doi.org/10.7554/eLife.45919

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