Cargando…
PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy
Cryo-electron tomography (cryo-ET) is emerging as a revolutionary method for resolving the structure of macromolecular complexes in situ. However, sample preparation for in situ Cryo-ET is labour-intensive and can require both cryo-lamella preparation through cryo-focused ion beam (FIB) milling and...
Autores principales: | Gorelick, Sergey, Buckley, Genevieve, Gervinskas, Gediminas, Johnson, Travis K, Handley, Ava, Caggiano, Monica Pia, Whisstock, James C, Pocock, Roger, de Marco, Alex |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609333/ https://www.ncbi.nlm.nih.gov/pubmed/31259689 http://dx.doi.org/10.7554/eLife.45919 |
Ejemplares similares
-
A modular platform for automated cryo-FIB workflows
por: Klumpe, Sven, et al.
Publicado: (2021) -
Direct detection pays off for electron cryo-microscopy
por: Grigorieff, Nikolaus
Publicado: (2013) -
Low cost, high performance processing of single particle cryo-electron microscopy data in the cloud
por: Cianfrocco, Michael A, et al.
Publicado: (2015) -
Molecular dynamics-based refinement and validation for sub-5 Å cryo-electron microscopy maps
por: Singharoy, Abhishek, et al.
Publicado: (2016) -
Cryo-plasma FIB/SEM volume imaging of biological specimens
por: Dumoux, Maud, et al.
Publicado: (2023)