Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast

Clone collections of modified strains (“libraries”) are a major resource for systematic studies with the yeast Saccharomyces cerevisiae. Construction of such libraries is time-consuming, costly and confined to the genetic background of a specific yeast strain. To overcome these limitations, we prese...

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Detalles Bibliográficos
Autores principales: Buchmuller, Benjamin C., Herbst, Konrad, Meurer, Matthias, Kirrmaier, Daniel, Sass, Ehud, Levy, Emmanuel D., Knop, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609715/
https://www.ncbi.nlm.nih.gov/pubmed/31273196
http://dx.doi.org/10.1038/s41467-019-10816-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609715/
https://www.ncbi.nlm.nih.gov/pubmed/31273196
http://dx.doi.org/10.1038/s41467-019-10816-7

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