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Pooled clone collections by multiplexed CRISPR-Cas12a-assisted gene tagging in yeast
Clone collections of modified strains (“libraries”) are a major resource for systematic studies with the yeast Saccharomyces cerevisiae. Construction of such libraries is time-consuming, costly and confined to the genetic background of a specific yeast strain. To overcome these limitations, we prese...
Autores principales: | Buchmuller, Benjamin C., Herbst, Konrad, Meurer, Matthias, Kirrmaier, Daniel, Sass, Ehud, Levy, Emmanuel D., Knop, Michael |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609715/ https://www.ncbi.nlm.nih.gov/pubmed/31273196 http://dx.doi.org/10.1038/s41467-019-10816-7 |
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