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Dual usage of a stage-specific fluorescent reporter system based on a helper-dependent adenoviral vector to visualize osteogenic differentiation

We developed a reporter system that can be used in a dual manner in visualizing mature osteoblast formation. The system is based on a helper-dependent adenoviral vector (HDAdV), in which a fluorescent protein, Venus, is expressed under the control of the 19-kb human osteocalcin (OC) genomic locus. B...

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Detalles Bibliográficos
Autores principales: Sone, Takefumi, Shin, Masashi, Ouchi, Takehito, Sasanuma, Hiroki, Miyamoto, Arei, Ohte, Satoshi, Tsukamoto, Sho, Nakanishi, Mahito, Okano, Hideyuki, Katagiri, Takenobu, Mitani, Kohnosuke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609771/
https://www.ncbi.nlm.nih.gov/pubmed/31273280
http://dx.doi.org/10.1038/s41598-019-46105-y