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Dual usage of a stage-specific fluorescent reporter system based on a helper-dependent adenoviral vector to visualize osteogenic differentiation
We developed a reporter system that can be used in a dual manner in visualizing mature osteoblast formation. The system is based on a helper-dependent adenoviral vector (HDAdV), in which a fluorescent protein, Venus, is expressed under the control of the 19-kb human osteocalcin (OC) genomic locus. B...
Autores principales: | Sone, Takefumi, Shin, Masashi, Ouchi, Takehito, Sasanuma, Hiroki, Miyamoto, Arei, Ohte, Satoshi, Tsukamoto, Sho, Nakanishi, Mahito, Okano, Hideyuki, Katagiri, Takenobu, Mitani, Kohnosuke |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6609771/ https://www.ncbi.nlm.nih.gov/pubmed/31273280 http://dx.doi.org/10.1038/s41598-019-46105-y |
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