A genetically encoded probe for imaging nascent and mature HA-tagged proteins in vivo

To expand the toolbox of imaging in living cells, we have engineered a single-chain variable fragment binding the linear HA epitope with high affinity and specificity in vivo. The resulting probe, called the HA frankenbody, can light up in multiple colors HA-tagged nuclear, cytoplasmic, membrane, an...

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Detalles Bibliográficos
Autores principales: Zhao, Ning, Kamijo, Kouta, Fox, Philip D., Oda, Haruka, Morisaki, Tatsuya, Sato, Yuko, Kimura, Hiroshi, Stasevich, Timothy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610143/
https://www.ncbi.nlm.nih.gov/pubmed/31270320
http://dx.doi.org/10.1038/s41467-019-10846-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610143/
https://www.ncbi.nlm.nih.gov/pubmed/31270320
http://dx.doi.org/10.1038/s41467-019-10846-1

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