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Establishment and phenotyping of neurosphere cultures from primary neuroblastoma samples

Background: Primary cell culture using serum free media supplemented with growth factors has been used in a number of cancers to propagate primary cells with stem like properties, which form as spherical cellular aggregates. Methods: We systematically evaluated the capacity of freshly disaggregated...

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Detalles Bibliográficos
Autores principales: Barton, Jack, Pacey, Katherine, Jain, Neha, Kasia, Tessa, Edwards, Darren, Thevanesan, Christine, Straathof, Karin, Barone, Giuseppe, Anderson, John
Formato: Online Artículo Texto
Lenguaje:English
Publicado: F1000 Research Limited 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611133/
https://www.ncbi.nlm.nih.gov/pubmed/31316758
http://dx.doi.org/10.12688/f1000research.18209.1