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Establishment and phenotyping of neurosphere cultures from primary neuroblastoma samples
Background: Primary cell culture using serum free media supplemented with growth factors has been used in a number of cancers to propagate primary cells with stem like properties, which form as spherical cellular aggregates. Methods: We systematically evaluated the capacity of freshly disaggregated...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
F1000 Research Limited
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611133/ https://www.ncbi.nlm.nih.gov/pubmed/31316758 http://dx.doi.org/10.12688/f1000research.18209.1 |