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Unbiased screen of RNA tailing activities reveals a poly(UG) polymerase

Ribonucleotidyl transferases (rNTases) add non-templated ribonucleotides to diverse RNAs. We developed TRAID-Seq, a screening strategy in S. cerevisiae to identify sequences added to a reporter RNA at single-nucleotide resolution by overexpressing candidate enzymes from different organisms. The rNTa...

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Detalles Bibliográficos
Autores principales:	Preston, Melanie A., Porter, Douglas F., Chen, Fan, Buter, Natascha, Lapointe, Christopher P., Keles, Sunduz, Kimble, Judith, Wickens, Marvin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6613791/ https://www.ncbi.nlm.nih.gov/pubmed/30988468 http://dx.doi.org/10.1038/s41592-019-0370-6

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