Quantitative Echotextural Attributes of Pectoralis Major Muscles in Broiler Chickens: Physicochemical Correlates and Effects of Dietary Fat Source

SIMPLE SUMMARY: There is presently no method of predicting meat quality based on a single examination of birds before slaughter. That could potentially be accomplished with a computer-assisted analysis of ultrasonographic images. Hence, this experiment was designed to draw correlations between various physical and chemical properties of chicken pectoral muscles and pixel values (brightness elements) measured in corresponding ultrasonograms (a.k.a. echotexture). Our study revealed that echotextural characteristics of pectoral muscles in live birds were predictive of several important physical parameters (e.g., cutting force, hardness, and chewiness) as well as intramuscular fat and protein content. However, different dietary fats that caused changes in the chemical composition of chicken breast also affected the associations between muscle echotexture and its physicochemical properties. For example, no correlations with the chemical composition of the muscles could be found in birds fed with soybean oil. We concluded that ultrasonographic imaging combined with a computerized image analysis can offer significant benefits to the poultry industry and consumers. It can aid in livestock genetic selection and improvement programs as well as enhance the quality of poultry meat and meat products. However, more confirmatory studies are needed. ABSTRACT: This study examined the relationships among physicochemical properties and ultrasonographic image attributes of pectoralis major muscles in broiler chickens. Forty male Ross 308 chicks were randomly assigned to four equinumerous fat-supplementation groups (Group SO: soybean oil; Group FO: flax oil; Group SO + FO: soybean oil + flax oil; and Group BF: beef fat). Ultrasonograms of birds’ pectoral muscles were obtained just before slaughter at 6 weeks of age and were subjected to digital image analyses to determine the mean pixel intensity (MPI) and pixel heterogeneity values (standard deviation of numerical pixel values; MPH). A total of 2, 4, 2, and 6 significant correlations were recorded in Groups SO, FO, SO + FO, and BF, respectively; there were no correlations with the chemical composition of the muscles in Groups SO and SO + FO. The strongest correlations were found between muscle lightness (L*) and MPH in Group BF (physical characteristic; r = −0.82, p = 0.003), and between crude fat/protein content and MPI/MPH of pectoral the major muscles in Groups FO/BF (chemical characteristics; r = 0.72, p = 0.02). There exists a potential application of ultrasonographic imaging and computerized image analysis for predicting certain physicochemical properties of pectoralis major muscles in broiler chickens.