Bindel-PCR: a novel and convenient method for identifying CRISPR/Cas9-induced biallelic mutants through modified PCR using Thermus aquaticus DNA polymerase

We developed a novel and convenient method for rapidly identifying CRISPR/Cas9-based genome-edited biallelic knockout (KO) cells/individuals carrying insertions or deletions of a few nucleotides (indels) by performing PCR on genomic DNA samples under stringent conditions and low MgCl(2) concentratio...

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Detalles Bibliográficos
Autores principales: Sakurai, Takayuki, Kamiyoshi, Akiko, Takei, Norio, Watanabe, Satoshi, Sato, Masahiro, Shindo, Takayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617447/
https://www.ncbi.nlm.nih.gov/pubmed/31289302
http://dx.doi.org/10.1038/s41598-019-46357-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6617447/
https://www.ncbi.nlm.nih.gov/pubmed/31289302
http://dx.doi.org/10.1038/s41598-019-46357-8

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