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A fully automated sample-to-answer PCR system for easy and sensitive detection of dengue virus in human serum and mosquitos
BACKGROUND: The insulated isothermal PCR (iiPCR) technology enables consistent PCR amplification and detection in a simple heating device. A pan-dengue virus (DENV) RT-iiPCR, targeting the 5’ untranslated region, was validated previously on the semi-automated POCKIT combo system (involving separate...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6619671/ https://www.ncbi.nlm.nih.gov/pubmed/31291289 http://dx.doi.org/10.1371/journal.pone.0218139 |