Optimized PCR conditions minimizing the formation of chimeric DNA molecules from MPRA plasmid libraries

BACKGROUND: Massively parallel reporter assays (MPRAs) enable high-throughput functional evaluation of various DNA regulatory elements and their mutant variants. The assays are based on construction of highly diverse plasmid libraries containing two variable fragments, a region of interest (a sequen...

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Detalles Bibliográficos
Autores principales: Omelina, Evgeniya S., Ivankin, Anton V., Letiagina, Anna E., Pindyurin, Alexey V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6620194/
https://www.ncbi.nlm.nih.gov/pubmed/31291895
http://dx.doi.org/10.1186/s12864-019-5847-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6620194/
https://www.ncbi.nlm.nih.gov/pubmed/31291895
http://dx.doi.org/10.1186/s12864-019-5847-2

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