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Optimized PCR conditions minimizing the formation of chimeric DNA molecules from MPRA plasmid libraries
BACKGROUND: Massively parallel reporter assays (MPRAs) enable high-throughput functional evaluation of various DNA regulatory elements and their mutant variants. The assays are based on construction of highly diverse plasmid libraries containing two variable fragments, a region of interest (a sequen...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6620194/ https://www.ncbi.nlm.nih.gov/pubmed/31291895 http://dx.doi.org/10.1186/s12864-019-5847-2 |