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In Vitro Pre-validation of Gene Editing by CRISPR/Cas9 Ribonucleoprotein

BACKGROUND: The CRISPR/Cas9 genome editing system is a powerful and simple gene editing method. The format of the CRISPR components is one of the important factors in targeting efficiency. Compared to plasmid or mRNA (IVTs) format, using the CRISPR/Cas9 system as Cas9–crRNA–tracrRNA RNP format is mo...

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Detalles Bibliográficos
Autores principales: Mehravar, Maryam, Shirazi, Abolfazl, Mehrazar, Mohammad Mehdi, Nazari, Mahboobeh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Avicenna Research Institute 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626505/
https://www.ncbi.nlm.nih.gov/pubmed/31380000