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5′,8-Cyclopurine Lesions in DNA Damage: Chemical, Analytical, Biological, and Diagnostic Significance

Purine 5′,8-cyclo-2′-deoxynucleosides (cPu) are tandem-type lesions observed among the DNA purine modifications and identified in mammalian cellular DNA in vivo. These lesions can be present in two diasteroisomeric forms, 5′R and 5′S, for each 2′-deoxyadenosine and 2′-deoxyguanosine moiety. They are...

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Autores principales: Chatgilialoglu, Chryssostomos, Ferreri, Carla, Geacintov, Nicholas E., Krokidis, Marios G., Liu, Yuan, Masi, Annalisa, Shafirovich, Vladimir, Terzidis, Michael A., Tsegay, Pawlos S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628319/
https://www.ncbi.nlm.nih.gov/pubmed/31141888
http://dx.doi.org/10.3390/cells8060513
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author Chatgilialoglu, Chryssostomos
Ferreri, Carla
Geacintov, Nicholas E.
Krokidis, Marios G.
Liu, Yuan
Masi, Annalisa
Shafirovich, Vladimir
Terzidis, Michael A.
Tsegay, Pawlos S.
author_facet Chatgilialoglu, Chryssostomos
Ferreri, Carla
Geacintov, Nicholas E.
Krokidis, Marios G.
Liu, Yuan
Masi, Annalisa
Shafirovich, Vladimir
Terzidis, Michael A.
Tsegay, Pawlos S.
author_sort Chatgilialoglu, Chryssostomos
collection PubMed
description Purine 5′,8-cyclo-2′-deoxynucleosides (cPu) are tandem-type lesions observed among the DNA purine modifications and identified in mammalian cellular DNA in vivo. These lesions can be present in two diasteroisomeric forms, 5′R and 5′S, for each 2′-deoxyadenosine and 2′-deoxyguanosine moiety. They are generated exclusively by hydroxyl radical attack to 2′-deoxyribose units generating C5′ radicals, followed by cyclization with the C8 position of the purine base. This review describes the main recent achievements in the preparation of the cPu molecular library for analytical and DNA synthesis applications for the studies of the enzymatic recognition and repair mechanisms, their impact on transcription and genetic instability, quantitative determination of the levels of lesions in various types of cells and animal model systems, and relationships between the levels of lesions and human health, disease, and aging, as well as the defining of the detection limits and quantification protocols.
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spelling pubmed-66283192019-07-23 5′,8-Cyclopurine Lesions in DNA Damage: Chemical, Analytical, Biological, and Diagnostic Significance Chatgilialoglu, Chryssostomos Ferreri, Carla Geacintov, Nicholas E. Krokidis, Marios G. Liu, Yuan Masi, Annalisa Shafirovich, Vladimir Terzidis, Michael A. Tsegay, Pawlos S. Cells Review Purine 5′,8-cyclo-2′-deoxynucleosides (cPu) are tandem-type lesions observed among the DNA purine modifications and identified in mammalian cellular DNA in vivo. These lesions can be present in two diasteroisomeric forms, 5′R and 5′S, for each 2′-deoxyadenosine and 2′-deoxyguanosine moiety. They are generated exclusively by hydroxyl radical attack to 2′-deoxyribose units generating C5′ radicals, followed by cyclization with the C8 position of the purine base. This review describes the main recent achievements in the preparation of the cPu molecular library for analytical and DNA synthesis applications for the studies of the enzymatic recognition and repair mechanisms, their impact on transcription and genetic instability, quantitative determination of the levels of lesions in various types of cells and animal model systems, and relationships between the levels of lesions and human health, disease, and aging, as well as the defining of the detection limits and quantification protocols. MDPI 2019-05-28 /pmc/articles/PMC6628319/ /pubmed/31141888 http://dx.doi.org/10.3390/cells8060513 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Chatgilialoglu, Chryssostomos
Ferreri, Carla
Geacintov, Nicholas E.
Krokidis, Marios G.
Liu, Yuan
Masi, Annalisa
Shafirovich, Vladimir
Terzidis, Michael A.
Tsegay, Pawlos S.
5′,8-Cyclopurine Lesions in DNA Damage: Chemical, Analytical, Biological, and Diagnostic Significance
title 5′,8-Cyclopurine Lesions in DNA Damage: Chemical, Analytical, Biological, and Diagnostic Significance
title_full 5′,8-Cyclopurine Lesions in DNA Damage: Chemical, Analytical, Biological, and Diagnostic Significance
title_fullStr 5′,8-Cyclopurine Lesions in DNA Damage: Chemical, Analytical, Biological, and Diagnostic Significance
title_full_unstemmed 5′,8-Cyclopurine Lesions in DNA Damage: Chemical, Analytical, Biological, and Diagnostic Significance
title_short 5′,8-Cyclopurine Lesions in DNA Damage: Chemical, Analytical, Biological, and Diagnostic Significance
title_sort 5′,8-cyclopurine lesions in dna damage: chemical, analytical, biological, and diagnostic significance
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6628319/
https://www.ncbi.nlm.nih.gov/pubmed/31141888
http://dx.doi.org/10.3390/cells8060513
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