Cargando…

Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins

Porcine circovirus type 2 (PCV2) is the etiological agent of porcine circovirus diseases and porcine circovirus-associated diseases (PCVDs/PCVADs). However, the pathogenesis of PCV2 is not fully understood. We previously found that 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) is negativel...

Descripción completa

Detalles Bibliográficos
Autores principales: Ouyang, Ting, Niu, Guyu, Zhang, Yifang, Liu, Xiaohua, Zhang, Xinwei, Zhang, Shiqi, Geng, Yulu, Pang, Daxin, Ouyang, Hongsheng, Ren, Linzhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630565/
https://www.ncbi.nlm.nih.gov/pubmed/31212640
http://dx.doi.org/10.3390/v11060544
_version_ 1783435332004347904
author Ouyang, Ting
Niu, Guyu
Zhang, Yifang
Liu, Xiaohua
Zhang, Xinwei
Zhang, Shiqi
Geng, Yulu
Pang, Daxin
Ouyang, Hongsheng
Ren, Linzhu
author_facet Ouyang, Ting
Niu, Guyu
Zhang, Yifang
Liu, Xiaohua
Zhang, Xinwei
Zhang, Shiqi
Geng, Yulu
Pang, Daxin
Ouyang, Hongsheng
Ren, Linzhu
author_sort Ouyang, Ting
collection PubMed
description Porcine circovirus type 2 (PCV2) is the etiological agent of porcine circovirus diseases and porcine circovirus-associated diseases (PCVDs/PCVADs). However, the pathogenesis of PCV2 is not fully understood. We previously found that 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) is negatively associated with PCV2 infection in vitro and in vivo. HMGCR inhibits the early stages of PCV2 infection, while PCV2 infection induces the phosphorylation of HMGCR to inactivate the protein. In this study, we investigated the possibility that adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK), and protein phosphatase 2 (PP2A) participate in HMGCR-mediated inhibition of PCV2 infection and the interaction of porcine HMGCR with PCV2 proteins. The results showed that AMPK activity fluctuated in cells during the early stage of PCV2 infection, while PP2A had little effect on PCV2 infection and HMGCR activity. Furthermore, PCV2 infection may enhance or maintain the level of phosphorylated HMGCR by directly interacting with the protein in PK-15 cells. These findings may provide a better understanding of PCV2 pathogenesis, and HMGCR may be a novel PCV2 antiviral target.
format Online
Article
Text
id pubmed-6630565
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-66305652019-08-19 Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins Ouyang, Ting Niu, Guyu Zhang, Yifang Liu, Xiaohua Zhang, Xinwei Zhang, Shiqi Geng, Yulu Pang, Daxin Ouyang, Hongsheng Ren, Linzhu Viruses Article Porcine circovirus type 2 (PCV2) is the etiological agent of porcine circovirus diseases and porcine circovirus-associated diseases (PCVDs/PCVADs). However, the pathogenesis of PCV2 is not fully understood. We previously found that 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR) is negatively associated with PCV2 infection in vitro and in vivo. HMGCR inhibits the early stages of PCV2 infection, while PCV2 infection induces the phosphorylation of HMGCR to inactivate the protein. In this study, we investigated the possibility that adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK), and protein phosphatase 2 (PP2A) participate in HMGCR-mediated inhibition of PCV2 infection and the interaction of porcine HMGCR with PCV2 proteins. The results showed that AMPK activity fluctuated in cells during the early stage of PCV2 infection, while PP2A had little effect on PCV2 infection and HMGCR activity. Furthermore, PCV2 infection may enhance or maintain the level of phosphorylated HMGCR by directly interacting with the protein in PK-15 cells. These findings may provide a better understanding of PCV2 pathogenesis, and HMGCR may be a novel PCV2 antiviral target. MDPI 2019-06-11 /pmc/articles/PMC6630565/ /pubmed/31212640 http://dx.doi.org/10.3390/v11060544 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ouyang, Ting
Niu, Guyu
Zhang, Yifang
Liu, Xiaohua
Zhang, Xinwei
Zhang, Shiqi
Geng, Yulu
Pang, Daxin
Ouyang, Hongsheng
Ren, Linzhu
Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins
title Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins
title_full Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins
title_fullStr Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins
title_full_unstemmed Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins
title_short Porcine HMGCR Inhibits Porcine Circovirus Type 2 Infection by Directly Interacting with the Viral Proteins
title_sort porcine hmgcr inhibits porcine circovirus type 2 infection by directly interacting with the viral proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6630565/
https://www.ncbi.nlm.nih.gov/pubmed/31212640
http://dx.doi.org/10.3390/v11060544
work_keys_str_mv AT ouyangting porcinehmgcrinhibitsporcinecircovirustype2infectionbydirectlyinteractingwiththeviralproteins
AT niuguyu porcinehmgcrinhibitsporcinecircovirustype2infectionbydirectlyinteractingwiththeviralproteins
AT zhangyifang porcinehmgcrinhibitsporcinecircovirustype2infectionbydirectlyinteractingwiththeviralproteins
AT liuxiaohua porcinehmgcrinhibitsporcinecircovirustype2infectionbydirectlyinteractingwiththeviralproteins
AT zhangxinwei porcinehmgcrinhibitsporcinecircovirustype2infectionbydirectlyinteractingwiththeviralproteins
AT zhangshiqi porcinehmgcrinhibitsporcinecircovirustype2infectionbydirectlyinteractingwiththeviralproteins
AT gengyulu porcinehmgcrinhibitsporcinecircovirustype2infectionbydirectlyinteractingwiththeviralproteins
AT pangdaxin porcinehmgcrinhibitsporcinecircovirustype2infectionbydirectlyinteractingwiththeviralproteins
AT ouyanghongsheng porcinehmgcrinhibitsporcinecircovirustype2infectionbydirectlyinteractingwiththeviralproteins
AT renlinzhu porcinehmgcrinhibitsporcinecircovirustype2infectionbydirectlyinteractingwiththeviralproteins