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Optimized Nuclear Pellet Method for Extracting Next-Generation Sequencing Quality Genomic DNA from Fresh Leaf Tissue

Next-generation sequencing (NGS) is a revolutionary advancement allowing large-scale discovery of functional molecular markers that has many applications, including plant breeding. High-quality genomic DNA (gDNA) is a prerequisite for successful NGS library preparation and sequencing; however, few r...

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Detalles Bibliográficos
Autores principales: Rana, Md Masud, Aycan, Murat, Takamatsu, Takeshi, Kaneko, Kentaro, Mitsui, Toshiaki, Itoh, Kimiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6632156/
https://www.ncbi.nlm.nih.gov/pubmed/31242613
http://dx.doi.org/10.3390/mps2020054