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Optimization of DNA Extraction from Individual Sand Flies for PCR Amplification

Numerous protocols have been published for extracting DNA from phlebotomines. Nevertheless, their small size is generally an issue in terms of yield, efficiency, and purity, for large-scale individual sand fly DNA extractions when using traditional methods. Even though this can be circumvented with...

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Detalles Bibliográficos
Autores principales: Caligiuri, Lorena G., Sandoval, Adolfo E., Miranda, Jose C., Pessoa, Felipe A., Santini, María S., Salomón, Oscar D., Secundino, Nagila F. C., McCarthy, Christina B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6632178/
https://www.ncbi.nlm.nih.gov/pubmed/31164615
http://dx.doi.org/10.3390/mps2020036