A piggyBac-based toolkit for inducible genome editing in mammalian cells

We describe the development and application of a novel series of vectors that facilitate CRISPR-Cas9-mediated genome editing in mammalian cells, which we call CRISPR-Bac. CRISPR-Bac leverages the piggyBac transposon to randomly insert CRISPR-Cas9 components into mammalian genomes. In CRISPR-Bac, a s...

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Detalles Bibliográficos
Autores principales: Schertzer, Megan D., Thulson, Eliza, Braceros, Keean C.A., Lee, David M., Hinkle, Emma R., Murphy, Ryan M., Kim, Susan O., Vitucci, Eva C.M., Calabrese, J. Mauro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2019
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6633203/
https://www.ncbi.nlm.nih.gov/pubmed/31101683
http://dx.doi.org/10.1261/rna.068932.118

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6633203/
https://www.ncbi.nlm.nih.gov/pubmed/31101683
http://dx.doi.org/10.1261/rna.068932.118

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